DDIT4 [p Thr23, p Thr25] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29521

Novus Biologicals, part of Bio-Techne
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NBP2-29521
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DDIT4 Antibodies »

Product Summary for DDIT4 [p Thr23, p Thr25] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 conjugated to KLH. Accession # Q9NX09

Modification

p Thr23, p Thr25

Specificity

Specific for endogenous levels of the ~34 kDa DDIT4 phosphorylated at Thr23/25. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDIT4 [p Thr23, p Thr25] Antibody

Western Blot: DDIT4 [p Thr23, p Thr25] Antibody [NBP2-29521]

Western Blot: DDIT4 [p Thr23, p Thr25] Antibody [NBP2-29521]

Western Blot: DDIT4 [p Thr23, p Thr25] Antibody [NBP2-29521] - Western blot of human jurkat lysate showing specific immunolabeling of the ~34k REDD1 protein phosphorylated at Thr23/25 (control). Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).

Applications for DDIT4 [p Thr23, p Thr25] Antibody

Application
Recommended Usage

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDIT4

REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A-DDB1-ROC1-b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b (Katiyar et al, 2009).

Alternate Names

Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1

Entrez Gene IDs

54541 (Human)

Gene Symbol

DDIT4

UniProt

Q9NX09 (Human)

Additional DDIT4 Products

Product Documents for DDIT4 [p Thr23, p Thr25] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDIT4 [p Thr23, p Thr25] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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