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DDO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32684

Novus Biologicals, part of Bio-Techne
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NBP2-32684
NBP2-32684-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for DDO Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MLIPHTYPDTPIHTQKQWFRETFNHLFAIANSAEAGDAGVHLVSGWQIFQSTPTEEVPFWADVVLGFRKMTE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDO Antibody

Immunocytochemistry/ Immunofluorescence: DDO Antibody [NBP2-32684]

Immunocytochemistry/ Immunofluorescence: DDO Antibody [NBP2-32684]

Immunocytochemistry/Immunofluorescence: DDO Antibody [NBP2-32684] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684]

Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684]

Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684] - Staining of human lymph node.
Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684]

Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684]

Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for DDO Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDO

OXDD (D-aspartate oxidase) is a peroxisomal flavoprotein that selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found.

Alternate Names

aspartic oxidase, DASOX, D-aspartate oxidase, DDO-1, DDO-2, EC 1.4.3.1, FLJ45203

Gene Symbol

DDO

Additional DDO Products

Product Documents for DDO Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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