DDOST Antibody (OTI2B4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70561

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DDOST Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 131-378 of human DDOST (NP_005207) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDOST Antibody (OTI2B4) - Azide and BSA Free

Western Blot: DDOST Antibody (OTI2B4)Azide and BSA Free [NBP2-70561]

Western Blot: DDOST Antibody (OTI2B4)Azide and BSA Free [NBP2-70561]

Western Blot: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST.
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561]

Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561]

Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561]

Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561]

Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for DDOST Antibody (OTI2B4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDOST

DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq]

Alternate Names

advanced glycation endproduct receptor 1, DDOST 48 kDa subunit, dolichyl-diphosphooligosaccharide-protein glycosyltransferase, dolichyl-diphosphooligosaccharide--protein glycosyltransferase, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, dolichyl-diphosphooligosaccharide-protein glycotransferase, EC 2.4.1.119, KIAA0115MGC2191, Oligosaccharyl transferase 48 kDa subunit, oligosaccharyltransferase 48 kDa subunit, OST48AGER1, OSTOKSWcl45, WBP1

Gene Symbol

DDOST

Additional DDOST Products

Product Documents for DDOST Antibody (OTI2B4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDOST Antibody (OTI2B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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