DDOST Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69562

Novus Biologicals, part of Bio-Techne
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NBP1-69562
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all DDOST Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to DDOST(dolichyl-diphosphooligosaccharide-protein glycosyltransferase) The peptide sequence was selected from the N terminal of DDOST. Peptide sequence SPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFD. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for DDOST Antibody

Western Blot: DDOST Antibody [NBP1-69562]

Western Blot: DDOST Antibody [NBP1-69562]

Western Blot: DDOST Antibody [NBP1-69562] - This Anti-DDOST antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 5ug/ml.

Applications for DDOST Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDOST

DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Alternate Names

advanced glycation endproduct receptor 1, DDOST 48 kDa subunit, dolichyl-diphosphooligosaccharide-protein glycosyltransferase, dolichyl-diphosphooligosaccharide--protein glycosyltransferase, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, dolichyl-diphosphooligosaccharide-protein glycotransferase, EC 2.4.1.119, KIAA0115MGC2191, Oligosaccharyl transferase 48 kDa subunit, oligosaccharyltransferase 48 kDa subunit, OST48AGER1, OSTOKSWcl45, WBP1

Gene Symbol

DDOST

UniProt

Q5VWA5 (Human)

Additional DDOST Products

Product Documents for DDOST Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDOST Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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