DDR2 Antibody (9A9)

Western Blot: DDR2 Antibody (9A9) [NBP3-26628] -

Western Blot: DDR2 Antibody (9A9) [NBP3-26628] - Positive Western Blot detected in: A549 whole cell lysate, SY5Y whole cell lysate, A375 whole cell lysate, HEPG2 whole cell lysate, Mouse Lung tissue lysate.
All lanes: DDR2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 96 kDa
Observed band size: 110 kDa

Flow Cytometry: DDR2 Antibody (9A9) [NBP3-26628] -

Flow Cytometry: DDR2 Antibody (9A9) [NBP3-26628] - Overlay Peak curve showing A549 cells stained with DDR2 Antibody (9A9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.