Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DDT Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DDT(NP_001346) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDT Antibody (OTI1H3) - Azide and BSA Free

Western Blot: DDT Antibody (OTI1H3)Azide and BSA Free [NBP2-70562]

Western Blot: DDT Antibody (OTI1H3)Azide and BSA Free [NBP2-70562]

Western Blot: DDT Antibody (OTI1H3) - Azide and BSA Free [NBP2-70562] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT.
Immunohistochemistry: DDT Antibody (OTI1H3) - Azide and BSA Free [NBP2-70562]

Immunohistochemistry: DDT Antibody (OTI1H3) - Azide and BSA Free [NBP2-70562]

Immunohistochemistry: DDT Antibody (OTI1H3) - Azide and BSA Free [NBP2-70562] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: DDT Antibody (OTI1H3)Azide and BSA Free [NBP2-70562]

Western Blot: DDT Antibody (OTI1H3)Azide and BSA Free [NBP2-70562]

Western Blot: DDT Antibody (OTI1H3) - Azide and BSA Free [NBP2-70562] - Analysis of extracts (35ug) from 9 different cell lines by using DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for DDT Antibody (OTI1H3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:200-8000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDT

D-dopachrome decarboxylase, also known as D-dopachrome tautomerase (DDT), phenylpyruvate tautomerase II and DDCT, is a member of the migration inhibitory factor (MIF) family. DDT, via tautomerization and decarboxylation, converts D-dopachrome into 5,6-dihydroxyindole (DHI). DDT may also play a role in melanin biosynthesis.

Long Name

D-Dopachrome Tautomerase

Alternate Names

D-Dopachrome Decarboxylase, DDCT, DOPD, MIF2, Phenylpyruvate Tautomerase II

Gene Symbol

DDT

Additional DDT Products

Product Documents for DDT Antibody (OTI1H3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDT Antibody (OTI1H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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