DDX19A/B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68149

Novus Biologicals, part of Bio-Techne
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NB100-68149
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DDX19A/B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLPVDKDGNPDNETY corresponding to internal region according to NP_001014451.1, NP_001014449.1, NP_060802.1.

Epitope

C-DLPVDKDGNPDNETY

Specificity

This antibody is expected to recognise DDX19A (NP_060802.1) and all reported isoforms of DDX19B (NP_009173.1, NP_001014451.1 and NP_001014449.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DDX19A/B Antibody

Western Blot: DDX19A/B Antibody [NB100-68149]

Western Blot: DDX19A/B Antibody [NB100-68149]

Western Blot: DDX19A/B Antibody [NB100-68149] - (0.5ug/ml) Analysis of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DDX19A/B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in lysates of human ovary and K562 (calculated MW of 50.5 kDa band according to NP_001014451.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDX19A/B

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

DDX19A DEAD (Asp-Glu-Ala-Asp) box polypeptide 19A, DDX19-DDX19L, DDX19L

Entrez Gene IDs

11269 (Human); 234733 (Mouse); 690693 (Rat)

Gene Symbol

DDX19A

UniProt

NP_009173.1 (Human)

Additional DDX19A/B Products

Product Documents for DDX19A/B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDX19A/B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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