DDX21 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1718

Novus Biologicals, part of Bio-Techne
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NB100-1718SS
NB100-1718
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human, Mouse, Porcine, Fish - Danio rerio (Zebrafish)

Predicted:

Canine (100%), Equine (100%), Porcine (100%), Primate (100%), Rat (100%), Rhesus Macaque (100%), Squirrel (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all DDX21 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 725 and the C-terminus (residue 783) of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, African elephant, Northern white-cheeked gibbon, and Thirteen-lined ground squirrel. Use in Zebrafish reported in scientific literature (PMID:32231306).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX21 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DDX21 Antibody - BSA Free [NB100-1718]

Immunocytochemistry/ Immunofluorescence: DDX21 Antibody - BSA Free [NB100-1718]

Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DDX21 Antibody NB100-1718 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: DDX21 Antibody - BSA Free [NB100-1718]

Flow Cytometry: DDX21 Antibody - BSA Free [NB100-1718]

Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on Jurkat Cells with DDX21Antibody NB100-1718 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).
Immunocytochemistry/ Immunofluorescence: DDX21 Antibody - BSA Free [NB100-1718]

Immunocytochemistry/ Immunofluorescence: DDX21 Antibody - BSA Free [NB100-1718]

Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with DDX21 Antibody conjugated to Alexa Fluor 488 (NB100-1718AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
View 12 more images

Applications for DDX21 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 32231306)

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2 - 10 ug/mg of lysate

Knockdown Validated

reported in scientific literature (PMID 35440492)

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 4 using NB100-1718 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX21

DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, DEAD box protein 21, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21, DKFZp686F21172, EC 3.6.1, EC 3.6.4.13, Gu protein, GUA, gu-alpha, GURDB, nucleolar RNA helicase 2, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/Gu, RH-II/GU, RH-II/GuA, RNA helicase II/Gu alpha

Entrez Gene IDs

9188 (Human); 56200 (Mouse)

Gene Symbol

DDX21

UniProt

Q9NR30 (Human)

Additional DDX21 Products

Product Documents for DDX21 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDX21 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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