Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DDX27 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 using the numbering given in entry NP_060365.6 (GeneID 55661).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX27 Antibody

Immunohistochemistry-Paraffin: DDX27 Antibody [NBP1-26601]

Immunohistochemistry-Paraffin: DDX27 Antibody [NBP1-26601]

Immunohistochemistry-Paraffin: DDX27 Antibody [NBP1-26601] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DDX27 used at a dilution of 1:5,000 ( 0.2ug/ml) and 1:1,000 ( 1ug/ml). Detection: DAB
Immunoprecipitation: DDX27 Antibody [NBP1-26601]

Immunoprecipitation: DDX27 Antibody [NBP1-26601]

Immunoprecipitation: DDX27 Antibody [NBP1-26601] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 10 mcg/mg lysate.

Applications for DDX27 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

5-10 ug/mg lysate
Application Notes
It is not recommended for western analysis. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX27

DDX27 (DEAD-box protein 27) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The function of DDX27 has not been characterized.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, DEAD box protein 27, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27, dJ686N3.1, DKFZp667N057, DRS1, EC 3.6.1, EC 3.6.4.13, FLJ12917, FLJ20596, FLJ22238, MGC1018, MGC163147, PP3241, probable ATP-dependent RNA helicase DDX27, RHLP, RNA helicase-like protein, Rrp3p

Entrez Gene IDs

55661 (Human)

Gene Symbol

DDX27

UniProt

NP_060365.6 (Human)

Additional DDX27 Products

Product Documents for DDX27 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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