DDX28 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83757

Novus Biologicals, part of Bio-Techne
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NBP1-83757-25ul
NBP1-83757
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX28 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TLLDESFLELVDYILEKSHIAEGPADLEDPFNPKAQLVLVGATFPEGVGQLLNKVASPDAVTTITSSKLHCIMPHVKQTFLRLKGADK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX28 Antibody

Western Blot: DDX28 Antibody [NBP1-83757]

Western Blot: DDX28 Antibody [NBP1-83757]

Western Blot: DDX28 Antibody [NBP1-83757] - Analysis in control (vector only transfected HEK293T lysate) and DDX28 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DDX28 Antibody [NBP1-83757]

Immunocytochemistry/ Immunofluorescence: DDX28 Antibody [NBP1-83757]

Immunocytochemistry/Immunofluorescence: DDX28 Antibody [NBP1-83757] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: DDX28 Antibody [NBP1-83757]

Immunohistochemistry-Paraffin: DDX28 Antibody [NBP1-83757]

Immunohistochemistry-Paraffin: DDX28 Antibody [NBP1-83757] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.

Applications for DDX28 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX28

DDX28, also known as Probable ATP-dependent RNA helicase DDX28, is a 59.6 kDa, 540 amino acid protein that is utilized in RNA processing and transport as a member of the DEAD box protein family. The protein is currently being studied for its involvement in prostatitis. As a part of the RNA binding pathway, the protein interacts with AARS2, ACAD9, ABCB7, ICT1, and AASS proteins.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 28, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28, EC 3.6.1, EC 3.6.4.13, FLJ11282, M, MDDX28probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28, mitochondrial DEAD-box polypeptide 28

Gene Symbol

DDX28

Additional DDX28 Products

Product Documents for DDX28 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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