DDX3 Antibody (11F11D10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14848

Novus Biologicals, part of Bio-Techne
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NBP2-14848
NBP2-14848SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 11F11D10

Format

BSA Free

Concentration

1.0 mg/ml

View all DDX3 Antibodies »

Product Specifications

Immunogen

A partial recombinant protein containing amino acids 24-203 of human DDX3 was used as immunogen for this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for DDX3 Antibody (11F11D10) - BSA Free

Western Blot: DDX3 Antibody (11F11D10)BSA Free [NBP2-14848]

Western Blot: DDX3 Antibody (11F11D10)BSA Free [NBP2-14848]

Western Blot: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: DDX3 Antibody (11F11D10) - BSA Free [NBP2-14848]

Flow Cytometry: DDX3 Antibody (11F11D10) - BSA Free [NBP2-14848]

Flow Cytometry: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of DDX3 in human MCF-7 and HeLa cells using DDX3 antibody at 1 ug/10^6 cells.

Applications for DDX3 Antibody (11F11D10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/10^6 cells

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX3

DDX3 is a conserved RNA helicase ubiquitously expressed in the cytoplasm. A 73kD protein and member of the DEAD-box family, DDX3 appears to have important regulatory activities in a variety of cell programs including growth and cell cycle control, apoptosis, and anti-viral signaling pathways. HIV and HCV co-opt the DDX3 molecule as an essential host factor for their replication. Additionally DDX3 appears to recognize and sense viral RNA, interacts with IPS-1 dependent signaling pathway molecules, and TRIF, an adaptor molecule in TLR3 and TLR4 signaling pathways, as well as ZBP1 which also is involved in upregulation of IFNb by HCMV. Loss of DDX3 may lead to enhanced proliferation and anti-apoptotic programs -pathways that promote a role for DDX3 in tumorigenesis. The availability of antibody specific for DDX3 provides opportunity to further detail its potential role in anti-viral immunity as well as serving as a novel target for intervention.

Alternate Names

DBXCAP-Rf, DDX14, DDX3DEAD/H box-3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, EC 3.6.1, EC 3.6.4.13, helicase like protein 2, Helicase-like protein 2, HLP2ATP-dependent RNA helicase DDX3X

Gene Symbol

DDX3X

UniProt

O00571 (Human)

Additional DDX3 Products

Product Documents for DDX3 Antibody (11F11D10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDX3 Antibody (11F11D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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