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DDX46 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83565

Novus Biologicals, part of Bio-Techne
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NBP1-83565
NBP1-83565-25ul

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for DDX46 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX46 Antibody

Western Blot: DDX46 Antibody [NBP1-83565]

Western Blot: DDX46 Antibody [NBP1-83565]

Western Blot: DDX46 Antibody [NBP1-83565] - Analysis using Anti-DDX46 antibody NBP1-83565 (A) shows similar pattern to independent antibody NBP2-56227 (B).
Immunohistochemistry-Paraffin: DDX46 Antibody [NBP1-83565]

Immunohistochemistry-Paraffin: DDX46 Antibody [NBP1-83565]

Immunohistochemistry-Paraffin: DDX46 Antibody [NBP1-83565] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.
Western Blot: DDX46 Antibody [NBP1-83565]

Western Blot: DDX46 Antibody [NBP1-83565]

Western Blot: DDX46 Antibody [NBP1-83565] - Analysis in human cell line HEK 293.

Applications for DDX46 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX46

DDX46 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, DEAD box protein 46, EC 3.6.1, EC 3.6.4.13, FLJ25329, KIAA0801probable ATP-dependent RNA helicase DDX46, MGC9936, Prp5, PRP5 homolog, Prp5-like DEAD-box protein, PRPF5, RNA helicase

Gene Symbol

DDX46

Additional DDX46 Products

Product Documents for DDX46 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDX46 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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