DDX49 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47491

Novus Biologicals, part of Bio-Techne
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NBP2-47491
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX49 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TLVTQYDIHLVHAIEEQIKKKLEEFSVEEAEVLQILTQVNVVRRECEIKLEAAHFDEKKEINKRKQLILEGKDPDLEAKRKAELAKIKQKN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX49 Antibody

Western Blot: DDX49 Antibody [NBP2-47491]

Western Blot: DDX49 Antibody [NBP2-47491]

Western Blot: DDX49 Antibody [NBP2-47491] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: DDX49 Antibody [NBP2-47491]

Immunocytochemistry/ Immunofluorescence: DDX49 Antibody [NBP2-47491]

Immunocytochemistry/Immunofluorescence: DDX49 Antibody [NBP2-47491] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria.
Immunohistochemistry-Paraffin: DDX49 Antibody [NBP2-47491]

Immunohistochemistry-Paraffin: DDX49 Antibody [NBP2-47491]

Immunohistochemistry-Paraffin: DDX49 Antibody [NBP2-47491] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

Applications for DDX49 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX49

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 49, DEAD box protein 49, EC 3.6.1, EC 3.6.4.13, FLJ10432, probable ATP-dependent RNA helicase DDX49, R27090_2

Gene Symbol

DDX49

Additional DDX49 Products

Product Documents for DDX49 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX49 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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