DDX50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60676

Novus Biologicals, part of Bio-Techne
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NB100-60676
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DDX50 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 687 and 737 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 using the numbering given in entry NP_076950.1 (GeneID 79009).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX50 Antibody

Western Blot: DDX50 Antibody [NB100-60676]

Western Blot: DDX50 Antibody [NB100-60676]

Western Blot: DDX50 Antibody [NB100-60676] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.04 ug/ml.

Applications for DDX50 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX50

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

Alternate Names

ATP-dependent RNA helicase DDX50, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50, DEAD box protein 50, EC 3.6.1, EC 3.6.4.13, GU2, GUB, gu-beta, MGC3199, Nucleolar protein Gu2, RH-II/GuB, RNA helicase II/Gu beta

Entrez Gene IDs

79009 (Human)

Gene Symbol

DDX50

UniProt

NP_076950.1 (Human)

Additional DDX50 Products

Product Documents for DDX50 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDX50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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