DDX50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83311

Novus Biologicals, part of Bio-Techne
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NBP1-83311
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX50 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX50 Antibody

Western Blot: DDX50 Antibody [NBP1-83311]

Western Blot: DDX50 Antibody [NBP1-83311]

Western Blot: DDX50 Antibody [NBP1-83311] - Analysis in control (vector only transfected HEK293T lysate) and DDX50 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DDX50 Antibody [NBP1-83311]

Immunocytochemistry/ Immunofluorescence: DDX50 Antibody [NBP1-83311]

Immunocytochemistry/Immunofluorescence: DDX50 Antibody [NBP1-83311] - Staining of human cell line A-431 shows positivity in nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DDX50 Antibody [NBP1-83311]

Immunohistochemistry-Paraffin: DDX50 Antibody [NBP1-83311]

Immunohistochemistry-Paraffin: DDX50 Antibody [NBP1-83311] - Staining of human vulva/anal skin shows moderate nucleolar positivity in squamous epithelial cells.

Applications for DDX50 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX50

DDX50, also known as ATP-dependent RNA helicase DDX50, is an 82.6 kDa 737 amino acid protein and is involved in cellular growth and division as a member of the DEAD box protein family. The protein is being studied for involvement in malaria. While involved in ATP and RNA binding pathways, the protein interacts with other proteins such as MED19, SRRM2, UBC, ESR2, and STAU1.

Alternate Names

ATP-dependent RNA helicase DDX50, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50, DEAD box protein 50, EC 3.6.1, EC 3.6.4.13, GU2, GUB, gu-beta, MGC3199, Nucleolar protein Gu2, RH-II/GuB, RNA helicase II/Gu beta

Gene Symbol

DDX50

Additional DDX50 Products

Product Documents for DDX50 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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