DDX54 Antibody (5B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00079039-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX54 Antibodies »

Product Specifications

Immunogen

DDX54 (NP_076977, 778 a.a. ~ 881 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDRDSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLHFLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM

Specificity

DDX54 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 (5B3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DDX54 Antibody (5B3)

Western Blot: DDX54 Antibody (5B3) [H00079039-M03]

Western Blot: DDX54 Antibody (5B3) [H00079039-M03]

Western Blot: DDX54 Antibody (5B3) [H00079039-M03] - Analysis of DDX54 expression in A-431 (Cat # L015V1).
Sandwich ELISA: DDX54 Antibody (5B3) [H00079039-M03] - Detection limit for recombinant GST tagged DDX54 is approximately 0.3ng/ml as a capture antibody.

Applications for DDX54 Antibody (5B3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDX54

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

APR-5, ATP-dependent RNA helicase DP97, DEAD (Asp-Glu-Ala-Asp) box polypeptide 54, DEAD box helicase 97 KDa, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97ATP-dependent RNA helicase DDX54, EC 3.6.1, EC 3.6.4.13, MGC2835

Entrez Gene IDs

79039 (Human)

Gene Symbol

DDX54

UniProt

NP_076977 (Human)

Additional DDX54 Products

Product Documents for DDX54 Antibody (5B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX54 Antibody (5B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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