DDX54 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60677

Novus Biologicals, part of Bio-Techne
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NB100-60677
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DDX54 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 625 and 675 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 using the numbering given in entry NP_076977.3 (GeneID 79039).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX54 Antibody

Western Blot: DDX54 Antibody [NB100-60677]

Western Blot: DDX54 Antibody [NB100-60677]

Western Blot: DDX54 Antibody [NB100-60677] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DDX54 antibody NB100-60677 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. DDX54 was also immunoprecipitated by rabbit anti-DDX54 antibody NB100-60678, which recognizes a downstream epitope.
Immunohistochemistry-Paraffin: DDX54 Antibody [NB100-60677]

Immunohistochemistry-Paraffin: DDX54 Antibody [NB100-60677]

Immunohistochemistry-Paraffin: DDX54 Antibody [NB100-60677] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Applications for DDX54 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX54

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Alternate Names

APR-5, ATP-dependent RNA helicase DP97, DEAD (Asp-Glu-Ala-Asp) box polypeptide 54, DEAD box helicase 97 KDa, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97ATP-dependent RNA helicase DDX54, EC 3.6.1, EC 3.6.4.13, MGC2835

Entrez Gene IDs

79039 (Human)

Gene Symbol

DDX54

UniProt

NP_076977.3 (Human)

Additional DDX54 Products

Product Documents for DDX54 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX54 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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