DDX6 Antibody (3D2)

Novus Biologicals, part of Bio-Techne | Catalog # H00001656-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX6 Antibodies »

Product Specifications

Immunogen

DDX6 (AAH65007, 1 a.a. ~ 483 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP

Specificity

DDX6 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DDX6 Antibody (3D2)

Western Blot: DDX6 Antibody (3D2) [H00001656-M01]

Western Blot: DDX6 Antibody (3D2) [H00001656-M01]

Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.Lane 1: DDX6 transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: DDX6 Antibody (3D2) [H00001656-M01]

Immunohistochemistry-Paraffin: DDX6 Antibody (3D2) [H00001656-M01]

Immunohistochemistry-Paraffin: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: DDX6 Antibody (3D2) [H00001656-M01]

Western Blot: DDX6 Antibody (3D2) [H00001656-M01]

Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi ( Cat # H00001656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # H00001656-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
View 1 more images

Applications for DDX6 Antibody (3D2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDX6

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

Alternate Names

ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), EC 3.6.1, EC 3.6.4.13, FLJ36338, HLR2DEAD box-6, Oncogene RCK, probable ATP-dependent RNA helicase DDX6, RCKP54

Entrez Gene IDs

1656 (Human)

Gene Symbol

DDX6

OMIM

600326 (Human)

UniProt

AAH65007 (Human)

Additional DDX6 Products

Product Documents for DDX6 Antibody (3D2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDX6 Antibody (3D2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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