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DEAF1 Antibody (1H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00010522-M04

Novus Biologicals, part of Bio-Techne
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H00010522-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DEAF1 (NP_066288.2, 133 a.a. ~ 222 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSG

Reactivity Notes

Human. Other species not tested.

Specificity

DEAF1 (1H8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DEAF1 Antibody (1H8)

Western Blot: DEAF1 Antibody (1H8) [H00010522-M04]

Western Blot: DEAF1 Antibody (1H8) [H00010522-M04]

Western Blot: DEAF1 Antibody (1H8) [H00010522-M04] - Analysis of DEAF1 expression in human uterus myoma.
Sandwich ELISA: DEAF1 Antibody (1H8) [H00010522-M04] - Detection limit for recombinant GST tagged DEAF1 is 0.3 ng/ml as a capture antibody.

Applications for DEAF1 Antibody (1H8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against tissue lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DEAF1

Transcription factor that binds to sequence with multiple copies of TTC[CG]G present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) AGGGTTCACCGAAAGTTCA. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase

Alternate Names

deformed epidermal autoregulatory factor 1 (Drosophila), Nuclear DEAF-1-related transcriptional regulator, NUDRdeformed epidermal autoregulatory factor 1 homolog, SPNsuppressin, Zinc finger MYND domain-containing protein 5, ZMYND5Suppressin

Entrez Gene IDs

10522 (Human)

Gene Symbol

DEAF1

OMIM

602635 (Human)

Additional DEAF1 Products

Product Documents for DEAF1 Antibody (1H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DEAF1 Antibody (1H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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