DEC1 Antibody (PSH01-91)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32947
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-91
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human DEC1 aa 151-412 / 412. (Uniprot: O14503)
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DEC1 Antibody (PSH01-91)
Western Blot: DEC1 Antibody (PSH01-91) [NBP3-32947]
Western Blot: DEC1 Antibody (PSH01-91) [NBP3-32947] - Western blot analysis of 45627 on different lysates with Rabbit anti-45627 antibody (NBP3-32947) at 1/1,000 dilution.Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: 293T cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32947) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for DEC1 Antibody (PSH01-91)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DEC1
Alternate Names
basic helix-loop-helix domain containing, class B, 2, basic helix-loop-helix family, member e40, bHLHb2, bHLHe40, Class B basic helix-loop-helix protein 2, class E basic helix-loop-helix protein 40, DEC1HLHB2, differentially expressed in chondrocytes 1, Differentially expressed in chondrocytes protein 1, differentiated embryo chondrocyte expressed gene 1, Enhancer-of-split and hairy-related protein 2, SHARP2, SHARP-2, Stimulated by retinoic acid gene 13 protein, STRA13FLJ99214, Stra14
Gene Symbol
BHLHE40
Additional DEC1 Products
Product Documents for DEC1 Antibody (PSH01-91)
Product Specific Notices for DEC1 Antibody (PSH01-91)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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