DEC1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47488
![Western Blot: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Western-Blot-NBP2-47488-img0005.jpg "Western Blot: DEC1 Antibody [NBP2-47488]")
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for DEC1 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GRRFTMGERIGAIKQESEEPPTKKNRMQLSDDEGHFTSSDLISSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPVLYPGLNASAAALSSFMNPDKISAPLLMPQRLPSPLPAHPSVDSSVLLQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DEC1 Antibody
![Western Blot: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Western-Blot-NBP2-47488-img0006.jpg "Western Blot: DEC1 Antibody [NBP2-47488]")
Western Blot: DEC1 Antibody [NBP2-47488]
Western Blot: DEC1 Antibody [NBP2-47488] - Analysis in control (vector only transfected HEK293T lysate) and BHLHE40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47488-img0004.jpg "Immunocytochemistry/ Immunofluorescence: DEC1 Antibody [NBP2-47488]")
Immunocytochemistry/ Immunofluorescence: DEC1 Antibody [NBP2-47488]
Immunocytochemistry/Immunofluorescence: DEC1 Antibody [NBP2-47488] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0011.jpg "Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]")
Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]
Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488] - Staining of human urinary bladder shows moderate to strong nuclear positivity in urothelial cells.![Immunohistochemistry: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunohistochemistry-NBP2-47488-img0001.jpg "Immunohistochemistry: DEC1 Antibody [NBP2-47488]")
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0007.jpg "Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]")
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0008.jpg "Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]")
![Immunohistochemistry: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunohistochemistry-NBP2-47488-img0009.jpg "Immunohistochemistry: DEC1 Antibody [NBP2-47488]")
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]](https://resources.bio-techne.com/images/products/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0010.jpg "Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488]")
Applications for DEC1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DEC1
Alternate Names
basic helix-loop-helix domain containing, class B, 2, basic helix-loop-helix family, member e40, bHLHb2, bHLHe40, Class B basic helix-loop-helix protein 2, class E basic helix-loop-helix protein 40, DEC1HLHB2, differentially expressed in chondrocytes 1, Differentially expressed in chondrocytes protein 1, differentiated embryo chondrocyte expressed gene 1, Enhancer-of-split and hairy-related protein 2, SHARP2, SHARP-2, Stimulated by retinoic acid gene 13 protein, STRA13FLJ99214, Stra14
Gene Symbol
BHLHE40
Additional DEC1 Products
Product Documents for DEC1 Antibody
Product Specific Notices for DEC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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