DEF6 Antibody (OTI3F9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71868

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DEF6 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

73.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DEF6 Antibody (OTI3F9) - Azide and BSA Free

Western Blot: DEF6 Antibody (OTI3F9)Azide and BSA Free [NBP2-71868]

Western Blot: DEF6 Antibody (OTI3F9)Azide and BSA Free [NBP2-71868]

Western Blot: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF6.
Immunohistochemistry: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868]

Immunohistochemistry: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868]

Immunohistochemistry: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868] - Analysis of Human pancreas tissue.
Immunohistochemistry: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868]

Immunohistochemistry: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868]

Immunohistochemistry: DEF6 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71868] - Analysis of Human bladder tissue.
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Applications for DEF6 Antibody (OTI3F9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DEF6

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Alternate Names

DEF-6, differentially expressed in FDCP 6 homolog, differentially expressed in FDCP 6 homolog (mouse), IBPdifferentially expressed in FDCP (mouse homolog) 6, IRF4-binding protein, SLAT, SWAP70L, SWAP-70-like adaptor protein of T cells

Gene Symbol

DEF6

Additional DEF6 Products

Product Documents for DEF6 Antibody (OTI3F9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DEF6 Antibody (OTI3F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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