DEF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81618

Novus Biologicals, part of Bio-Techne
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NBP1-81618
NBP1-81618-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DEF6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RADEDVEAAQRKLRQASTNVKHWNVQMNRLMHPIEPGDKRPVTSSSFSGFQPPLLAHRDSSLKRLTRWGSQGNRTPSPNSNEQQKSLNGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DEF6 Antibody

Western Blot: DEF6 Antibody [NBP1-81618]

Western Blot: DEF6 Antibody [NBP1-81618]

Western Blot: DEF6 Antibody [NBP1-81618] - Analysis in human cell lines A-431 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618]

Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618]

Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining in human lymph node and kidney tissues using anti-DEF6 antibody. Corresponding DEF6 RNA-seq data are presented for the same tissues.
Western Blot: DEF6 Antibody [NBP1-81618]

Western Blot: DEF6 Antibody [NBP1-81618]

Western Blot: DEF6 Antibody [NBP1-81618] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
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Applications for DEF6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEF6

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Alternate Names

DEF-6, differentially expressed in FDCP 6 homolog, differentially expressed in FDCP 6 homolog (mouse), IBPdifferentially expressed in FDCP (mouse homolog) 6, IRF4-binding protein, SLAT, SWAP70L, SWAP-70-like adaptor protein of T cells

Gene Symbol

DEF6

Additional DEF6 Products

Product Documents for DEF6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DEF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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