Defensin beta 1 Antibody (1F5)

Flow Cytometry: Defensin beta 1 Antibody (1F5) [NBP3-26625] -

Flow Cytometry: Defensin beta 1 Antibody (1F5) [NBP3-26625] - Overlay Peak curve showing HepG2 cells stained with Defensin beta 1 Antibody (1F5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.