Defensin beta 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11287

Novus Biologicals, part of Bio-Techne
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NBP3-11287
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

0.1 mg/ml

View all Defensin beta 2 Antibodies »

Product Specifications

Immunogen

CHPVFCPRRYKQIGTCGL

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Defensin beta 2 Antibody

Immunohistochemistry-Paraffin: Defensin beta 2 Antibody [NBP3-11287]

Immunohistochemistry-Paraffin: Defensin beta 2 Antibody [NBP3-11287]

Immunohistochemistry-Paraffin: Defensin beta 2 Antibody [NBP3-11287] - Submucosa of a bronchus with hBD-2 positive glands (rabbit 2, 1/200, Citrate).

Applications for Defensin beta 2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry-paraffin embedded sections and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Defensin beta 2

The polyclonal antibody recognizes human beta-defensin 2 (hBD-2). hBD-2 is a cystein-rich cationic 41 amino acid antimicrobial peptide of 4-5 kDa. Human BD-2 is produced by epithelial cells upon stimulus by lipopolysaccharides and proinflammatory cytokines TNFalpha and IL1beta. Contact of keratinocytes with gram-negative bacteria results in rapid induction of hBD-2 protein. hBD-2 has been described as a dynamic component of the local epithelial defense system of the skin, intestinal and respiratory tract, where it functions by protecting surfaces from infection. Its local expression has been associated with skin lesions like psoriasis as well as infected lung epithelia of patients with cystic fibrosis. Furthermore, in inflammatory bowel disease (IBD), expression of hBD-2 is increased in patients with IBD compared to healthy persons. The NF-kB pathway has been recognized as a key component in the induction of hBD-2 expression, however, other studies have observed induction mediated by the mitogen-activated protein kinase (MAPK) pathways. Thus, increased expression of hBD-2 in epithelial cells is associated with the proinflammatory response. This is supported by the finding that the anti-inflammatory cytokines IL-10 and IL-13 downregulate the synthesis of hBD-2 in atopic dermatitis.

Alternate Names

BD-2, Defb2, Defb2 defensin beta 2, defensin beta 2

Gene Symbol

Defb2

Additional Defensin beta 2 Products

Product Documents for Defensin beta 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Defensin beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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