DEGS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12689

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DEGS1 Antibodies »

Product Summary for DEGS1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human DEGS1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for DEGS1 Antibody

Western Blot: DEGS1 Antibody [NBP3-12689]

Western Blot: DEGS1 Antibody [NBP3-12689]

Western Blot: DEGS1 Antibody [NBP3-12689] - Lane A: 293T Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: U87-MG Whole Cell LysateLane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:38 kDaObserved band size:34 kDa
Immunoprecipitation: DEGS1 Antibody [NBP3-12689]

Immunoprecipitation: DEGS1 Antibody [NBP3-12689]

Immunoprecipitation: DEGS1 Antibody [NBP3-12689] - Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-DEGS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-DEGS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size :34 kDa

Applications for DEGS1 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEGS1

DEGS1 encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. Two splice variants have been identified. [provided by RefSeq]

Long Name

Sphingolipid delta(4)-desaturase DES1

Alternate Names

DES1, EC 1.14.19.17, EC 5.2.1.-, MIG15, Retinol isomerase

Gene Symbol

DEGS1

Additional DEGS1 Products

Product Documents for DEGS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DEGS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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