DEM1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88493
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for DEM1 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:ELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFGEVEGVLLVGVIDELHYTAKG
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DEM1 Antibody
![Western Blot: DEM1 Antibody [NBP1-88493]](https://resources.bio-techne.com/images/products/DEM1-Antibody-Western-Blot-NBP1-88493-img0006.jpg "Western Blot: DEM1 Antibody [NBP1-88493]")
Western Blot: DEM1 Antibody [NBP1-88493]
Western Blot: DEM1 Antibody [NBP1-88493] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
![Immunocytochemistry/ Immunofluorescence: DEM1 Antibody [NBP1-88493]](https://resources.bio-techne.com/images/products/DEM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88493-img0007.jpg "Immunocytochemistry/ Immunofluorescence: DEM1 Antibody [NBP1-88493]")
Immunocytochemistry/ Immunofluorescence: DEM1 Antibody [NBP1-88493]
Immunocytochemistry/Immunofluorescence: DEM1 Antibody [NBP1-88493] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.Applications for DEM1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DEM1
Alternate Names
C1orf176, chromosome 1 open reading frame 176, defects in morphology 1 homolog (S. cerevisiae), Defects in morphology protein 1 homolog, EC 3.1.11.-, Exo V, FLJ11445, FLJ13183, FLJ21144
Gene Symbol
EXO5
Additional DEM1 Products
Product Documents for DEM1 Antibody
Product Specific Notices for DEM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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