Dengue virus 1 NS1 Antibody (BA11-D5-1-B11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14854

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Virus

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BA11-D5-1-B11

Concentration

1 mg/ml

View all Dengue Virus 1 NS1 Antibodies »

Product Specifications

Immunogen

An equal mix of Dengue virus NS1 from serotypes 1-4, produced in HEK293 cells

Reactivity Notes

Dengue Serotype 1

Specificity

This antibody recognises Dengue virus 1 NS1 protein. It shows no cross-reactivity in ELISA with NS1 from Dengue virus serotypes 2, 3 or 4, ZIKV, WSLV, TBEV, JEV, SLEV, POWV, USUV, WNV and YFV. In western blot it detects NS1 for all four Dengue virus serotypes and St. Louis encephalitis virus (SLEV) NS1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Addition of 0.09% w/v sodium azide is recommended for long term storage at +4C.

Scientific Data Images for Dengue virus 1 NS1 Antibody (BA11-D5-1-B11)

Western Blot: Dengue virus 1 NS1 Antibody (BA11-D5-1-B11) [NBP3-14854]

Western Blot: Dengue virus 1 NS1 Antibody (BA11-D5-1-B11) [NBP3-14854]

Western Blot: Dengue virus 1 NS1 Antibody (BA11-D5-1-B11) [NBP3-14854] - 100ng of each antigen was used for SDS-PAGE, in reduced form. Proteins were transferred using Transblot for 7 minutes at 25V. 5% dry milk in PBS-T was used as blocking buffer and dilution buffer for antibodies. Primary antibodies are given below, and goat anti-mouse-IgG-HRP secondary antibody was used at 1:1000. All steps were carried out for 1h at room temperature with gentle rocking. KPL Membrane TMB was used for detection. Development time 30 seconds. Antibody detected NS1 protein for all four Dengue virus serotypes and St. Louis encephalitis virus (SLEV).
ELISA: Dengue virus 1 NS1 Antibody (BA11-D5-1-B11) [NBP3-14854]

ELISA: Dengue virus 1 NS1 Antibody (BA11-D5-1-B11) [NBP3-14854]

ELISA: Dengue virus 1 NS1 Antibody (BA11-D5-1-B11) [NBP3-14854] - All NS1 antigens coated at 0.5ug/ml in DPBS for 2h at RT. Plate washed 1X 300ul/well TBS + 0.1% Tween20, blocked 300ul/well DPBS+1% BSA for 1h. Antibody diluted to 1.0ug/ml and 0.01ugml in DPBS + 1% BSA + 0.05% T20. Added at 100ul/well, incubated shaken 2h room temperature. Plate washed 3X 300ul/well TBS-T wash buffer. Goat anti-mouse IgG-HRP secondary antibody diluted 1:2500 in DPBS/1%BSA/0.05%T20, added at 100ul/well, incubated shaken 1h room temperature. Plate washed 6X 300ul/well TBS-T wash buffer. Detection by Europa TMB substrate, added at 100ul/well and the plate developed for 3 min. static on the bench. Reaction stopped with 100ul/well 1M HCL and the plate was read within 4 min. at 405nm.

Applications for Dengue virus 1 NS1 Antibody (BA11-D5-1-B11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH 7.4

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dengue Virus 1 NS1

Dengue virus is a single -stranded, positive-sense RNA virus belonging to the Flavivirdae family and is the causative agent of Dengue fever (1-3). Other viruses in the same family include Japanese encephalitis virus, West Nile virus, and yellow fever (2,3). The Dengue virus is primarily transmitted through Aedes mosquito bites, with 100-400 million people infected annually and approximately 20,000 deaths worldwide per year (1,3). There are four known Dengue virus serotypes (DENV 1-4) that share 65% amino acid sequence similarity (1-2). The serotypes can be further classified into genotypes according to geographical distribution (2,3). The structure of the Dengue virus is ~50 nanometers (nm) in diameter, spherical in shape, and consists of an enveloped single-stranded RNA, an icosahedral nucleocapsid core, and a lipid bilayer (1-3). The Dengue virus genome is 11-kilobases (kb) long and has a 5' untranslated region (UTR), an open reading frame (ORF), and a 3' UTR (1,3). The ORF encodes a polyprotein for the translation of the three structural proteins and seven non-structural proteins (1-3). The structural proteins are the capsid (100 amino acids (aa), 12 kilodalton (kDa)), the pre-membrane (166 aa) or membrane (75 aa, 8.2-8.5 kDa), and envelope (495 aa, 53 kDa) (1-3). The nonstructural proteins are NS1 (350 aa, 45 kDa), NS2A (218 aa, 22 kDa), NS2B (130 aa, 14 kDa), NS3 (618 aa, 70 kDa), NS4A (150 aa, 16 kDa), NS4B (245-249 aa, 27 kDa), NS5 (900 aa, 104 kDa) (1-3).

Dengue virus entry into host cells occurs via receptor-mediated endocytosis by receptor molecules including the mannose receptor, heparan sulfate, glycosaminoglycans, and DC-SIGN (1,3). Following attachment, the virus is endocytosed in clathrin-coated vesicles (1,3). Following internalization, clathrin disassembles and endosomal processing occurs, allowing viral fusion, disassembly, and release of viral RNA (1,3). This release results in viral translation and replication, virus assembly and maturation, and eventual exocytosis of the mature virus (1,3). Infection can result in a wide range of clinical symptoms including mild disease such as Dengue fever which is characterized by fever, headache, joint pain, rash, and retro-orbital pain, or severe, life-threatening conditions like Dengue hemorrhagic fever or Dengue shock syndrome which involves vascular permeability and leakage (1-3). Host immune response against infection includes innate immune response via interferon secretion and pro-inflammatory cytokine production, as well as adaptive immune response involving cellular and humoral components like T cell activation and B-cell mediated antibody production (1-3). As far as treatment for Dengue virus infection, there no commercial antiviral agents, though some anti-pyretics and certain phenolic compounds do show promise in treating infection (1-3). However, Resveratol, an antiviral for other Flavivirus, has been shown to directly attack the Dengue virus genome (1). While more work needs to be done, there are some live-attenuated tetravalent Dengue virus vaccine candidates in clinal trials including DENVax and TV003/TV005 (1-3).

References

1. Nanaware N, Banerjee A, Mullick Bagchi S, Bagchi P, Mukherjee A. Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses. Viruses. 2021;13(10):1967. Published 2021 Sep 30. https://doi.org/10.3390/v13101967

2. Harapan H, Michie A, Sasmono RT, Imrie A. Dengue: A Minireview. Viruses. 2020;12(8):829. Published 2020 Jul 30. https://doi.org/10.3390/v12080829

3. Roy SK, Bhattacharjee S. Dengue virus: epidemiology, biology, and disease aetiology. Can J Microbiol. 2021;67(10):687-702. https://doi.org/10.1139/cjm-2020-0572

Long Name

Dengue Virus 1 Nauru/West Pac/1974 Polyprotein

Alternate Names

DEN-1, Dengue virus Serotype 1, Dengue virus Type 1, DENV1, DENV1-NS1

Additional Dengue Virus 1 NS1 Products

Product Documents for Dengue virus 1 NS1 Antibody (BA11-D5-1-B11)

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Product Specific Notices for Dengue virus 1 NS1 Antibody (BA11-D5-1-B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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