Dengue Virus 2 Envelope Antibody (GT643)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13488

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Virus

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # GT643

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue Virus 2 Envelope (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Reactivity Notes

Dengue virus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Dengue Virus 2 Envelope Antibody (GT643)

Western Blot: Dengue Virus 2 Envelope Antibody (GT643) [NBP3-13488]

Western Blot: Dengue Virus 2 Envelope Antibody (GT643) [NBP3-13488] - Envelop protein (Dengue virus 2) antibody [GT643] detects Envelop protein (Dengue virus 2) protein by western blot analysis. A. 30 ug BHK21 whole cell lysate/extract B. 30 ug whole cell lysate/extract of Dengue virus type 2 infected BHK21 cells C. 30 ug whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells 12% SDS-PAGE Envelop protein (Dengue virus 2) antibody [GT643] (NBP3-13488) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Dengue Virus 2 Envelope Antibody (GT643) [NBP3-13488]

Immunohistochemistry-Paraffin: Dengue Virus 2 Envelope Antibody (GT643) [NBP3-13488] - Dengue Virus 2 Envelope antibody [GT643] detects Dengue Virus 2 Envelope protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dengue virus BHK-21. Red: Dengue Virus 2 Envelope stained by Dengue Virus 2 Envelope antibody [GT643] (NBP3-13488) diluted at 1:2000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Dengue Virus 2 Envelope Antibody (GT643) [NBP3-13488]

Immunocytochemistry/Immunofluorescence: Dengue Virus 2 Envelope Antibody (GT643) [NBP3-13488] - Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde. Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (NBP3-13488) diluted at 1:2000. Blue: Hoechst 33342 staining.

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Applications for Dengue Virus 2 Envelope Antibody (GT643)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dengue Virus 2 Envelope

Dengue virus is a single -stranded, positive-sense RNA virus belonging to the Flavivirdae family and is the causative agent of Dengue fever (1-3). Other viruses in the same family include Japanese encephalitis virus, West Nile virus, and yellow fever (2,3). The Dengue virus is primarily transmitted through Aedes mosquito bites, with 100-400 million people infected annually and approximately 20,000 deaths worldwide per year (1,3). There are four known Dengue virus serotypes (DENV 1-4) that share 65% amino acid sequence similarity (1-2). The serotypes can be further classified into genotypes according to geographical distribution (2,3). The structure of the Dengue virus is ~50 nanometers (nm) in diameter, spherical in shape, and consists of an enveloped single-stranded RNA, an icosahedral nucleocapsid core, and a lipid bilayer (1-3). The Dengue virus genome is 11-kilobases (kb) long and has a 5' untranslated region (UTR), an open reading frame (ORF), and a 3' UTR (1,3). The ORF encodes a polyprotein for the translation of the three structural proteins and seven non-structural proteins (1-3). The structural proteins are the capsid (100 amino acids (aa), 12 kilodalton (kDa)), the pre-membrane (166 aa) or membrane (75 aa, 8.2-8.5 kDa), and envelope (495 aa, 53 kDa) (1-3). The nonstructural proteins are NS1 (350 aa, 45 kDa), NS2A (218 aa, 22 kDa), NS2B (130 aa, 14 kDa), NS3 (618 aa, 70 kDa), NS4A (150 aa, 16 kDa), NS4B (245-249 aa, 27 kDa), NS5 (900 aa, 104 kDa) (1-3).

Dengue virus entry into host cells occurs via receptor-mediated endocytosis by receptor molecules including the mannose receptor, heparan sulfate, glycosaminoglycans, and DC-SIGN (1,3). Following attachment, the virus is endocytosed in clathrin-coated vesicles (1,3). Following internalization, clathrin disassembles and endosomal processing occurs, allowing viral fusion, disassembly, and release of viral RNA (1,3). This release results in viral translation and replication, virus assembly and maturation, and eventual exocytosis of the mature virus (1,3). Infection can result in a wide range of clinical symptoms including mild disease such as Dengue fever which is characterized by fever, headache, joint pain, rash, and retro-orbital pain, or severe, life-threatening conditions like Dengue hemorrhagic fever or Dengue shock syndrome which involves vascular permeability and leakage (1-3). Host immune response against infection includes innate immune response via interferon secretion and pro-inflammatory cytokine production, as well as adaptive immune response involving cellular and humoral components like T cell activation and B-cell mediated antibody production (1-3). As far as treatment for Dengue virus infection, there no commercial antiviral agents, though some anti-pyretics and certain phenolic compounds do show promise in treating infection (1-3). However, Resveratol, an antiviral for other Flavivirus, has been shown to directly attack the Dengue virus genome (1). While more work needs to be done, there are some live-attenuated tetravalent Dengue virus vaccine candidates in clinal trials including DENVax and TV003/TV005 (1-3).

References

1. Nanaware N, Banerjee A, Mullick Bagchi S, Bagchi P, Mukherjee A. Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses. Viruses. 2021;13(10):1967. Published 2021 Sep 30. https://doi.org/10.3390/v13101967

2. Harapan H, Michie A, Sasmono RT, Imrie A. Dengue: A Minireview. Viruses. 2020;12(8):829. Published 2020 Jul 30. https://doi.org/10.3390/v12080829

3. Roy SK, Bhattacharjee S. Dengue virus: epidemiology, biology, and disease aetiology. Can J Microbiol. 2021;67(10):687-702. https://doi.org/10.1139/cjm-2020-0572

Alternate Names

Dengue 2 envelope;Dengue envelope;envelope protein, Envelope protein E

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Product Specific Notices for Dengue Virus 2 Envelope Antibody (GT643)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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