DENND3 [p Ser554] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60771
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Primate (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 conjugated to KLH. Accession # D4A2H4
Modification
p Ser554
Specificity
Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DENND3 [p Ser554] Antibody
![Western Blot: DENND3 [p Ser554] Antibody [NBP2-60771]](https://resources.bio-techne.com/images/products/DENND3-[p-Ser554]-Antibody-Western-Blot-NBP2-60771-img0001.jpg "Western Blot: DENND3 [p Ser554] Antibody [NBP2-60771]")
Western Blot: DENND3 [p Ser554] Antibody [NBP2-60771]
Western Blot: DENND3 [p Ser554] Antibody [NBP2-60771] - Western blot of HeLa cell lysate showing specificimmunolabeling of the ~142 kDa DENND3 proteinphosphorylated at Ser554 in the first lane (-). Phosphospecificityis shown in the second lane (+) where immunolabeling iscompletely eliminated by lysate treatment with lambdaphosphatase (Lambda-Ptase, 800 units/1mg protein for 30 min).Applications for DENND3 [p Ser554] Antibody
Application
Recommended Usage
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DENND3
Alternate Names
DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3
Gene Symbol
DENND3
Additional DENND3 Products
Product Documents for DENND3 [p Ser554] Antibody
Product Specific Notices for DENND3 [p Ser554] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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