Deoxycytidylate deaminase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87424

Novus Biologicals, part of Bio-Techne
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NBP1-87424
NBP1-87424-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Deoxycytidylate deaminase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QPCGPNMSEVSCKKRDDYLEWPEYFMAVAFLSAQRSKDPNSQVGACIVNSENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYPYVCHA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Deoxycytidylate deaminase Antibody

Western Blot: Deoxycytidylate deaminase Antibody [NBP1-87424]

Western Blot: Deoxycytidylate deaminase Antibody [NBP1-87424]

Western Blot: Deoxycytidylate deaminase Antibody [NBP1-87424] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-160
Immunocytochemistry/ Immunofluorescence: Deoxycytidylate deaminase Antibody [NBP1-87424]

Immunocytochemistry/ Immunofluorescence: Deoxycytidylate deaminase Antibody [NBP1-87424]

Immunocytochemistry/Immunofluorescence: Deoxycytidylate deaminase Antibody [NBP1-87424] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody [NBP1-87424]

Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody [NBP1-87424]

Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody [NBP1-87424] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for Deoxycytidylate deaminase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Deoxycytidylate deaminase

Deoxycytidylate deaminase is encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

dCMP deaminaseEC 3.5.4.12, deoxycytidylate deaminase, MGC111062

Gene Symbol

DCTD

Additional Deoxycytidylate deaminase Products

Product Documents for Deoxycytidylate deaminase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Deoxycytidylate deaminase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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