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Deoxyguanosine kinase Antibody (3E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00001716-M02

Novus Biologicals, part of Bio-Techne
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H00001716-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DGUOK (NP_001920, 1 a.a. ~ 89 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAGRLFLSRLRAPFSSMAKSPLEGVSSSRGLHAGRGPRRLSIEGNIGLHCPKSWKLAGYDVPGASTMVLHIPDIFLFEPPESTAGALP

Specificity

DGUOK (3E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Deoxyguanosine kinase Antibody (3E9)

Western Blot: Deoxyguanosine kinase Antibody (3E9) [H00001716-M02]

Western Blot: Deoxyguanosine kinase Antibody (3E9) [H00001716-M02]

Western Blot: Deoxyguanosine kinase Antibody (3E9) [H00001716-M02] - Analysis of DGUOK expression in transfected 293T cell line by DGUOK monoclonal antibody (M02), clone 3E9.Lane 1: DGUOK transfected lysate(32.056 KDa).Lane 2: Non-transfected lysate.
Western Blot: Deoxyguanosine kinase Antibody (3E9) [H00001716-M02]

Western Blot: Deoxyguanosine kinase Antibody (3E9) [H00001716-M02]

Western Blot: Deoxyguanosine kinase Antibody (3E9) [H00001716-M02] - Analysis of DGUOK over-expressed 293 cell line, cotransfected with DGUOK Validated Chimera RNAi ( Cat # H00001716-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DGUOK monoclonal antibody (M02), clone 3E9 (Cat # H00001716-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for Deoxyguanosine kinase Antibody (3E9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Deoxyguanosine kinase

In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene.

Alternate Names

deoxyguanosine kinase, deoxyguanosine kinase, mitochondrial, DGK, dGKmitochondrial deoxyguanosine kinase, EC 2.7.1.113, MTDPS3

Entrez Gene IDs

1716 (Human)

Gene Symbol

DGUOK

OMIM

251880 (Human)

Additional Deoxyguanosine kinase Products

Product Documents for Deoxyguanosine kinase Antibody (3E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Deoxyguanosine kinase Antibody (3E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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