DEP-1/CD148 Antibody (6G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00005795-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DEP-1/CD148 Antibodies »

Product Specifications

Immunogen

PTPRJ (NP_002834.2, 38 a.a. ~ 137 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPEQGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVIL

Specificity

PTPRJ - protein tyrosine phosphatase, receptor type, J

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DEP-1/CD148 Antibody (6G11)

Sandwich ELISA: DEP-1/CD148 Antibody (6G11) [H00005795-M01] - Detection limit for recombinant GST tagged PTPRJ is approximately 0.03ng/ml as a capture antibody.

Applications for DEP-1/CD148 Antibody (6G11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DEP-1/CD148

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Alternate Names

CD148, DEP1, HPTP-eta, PTPRJ

Entrez Gene IDs

5795 (Human)

Gene Symbol

PTPRJ

OMIM

114 (Human)

UniProt

NP_002834 (Human)

Additional DEP-1/CD148 Products

Product Documents for DEP-1/CD148 Antibody (6G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DEP-1/CD148 Antibody (6G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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