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DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21790

Novus Biologicals, part of Bio-Techne
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NBP2-21790
Conjugate
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MEM-CD148/05

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Human recombinant CD148 (amino acids 1-444).

Specificity

The mouse monoclonal antibody MEM-CD148/05 recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free [NBP2-21790]

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free [NBP2-21790]

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) [NBP2-21790] - Surface staining of human peripheral blood leukocytes using anti-CD148 (MEM-CD148/05) PE.
Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free [NBP2-21790]

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free [NBP2-21790]

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) [NBP2-21790] - Surface staining of human peripheral blood using anti-CD148 (MEM-CD148/05) APC.
Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free [NBP2-21790]

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free [NBP2-21790]

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) [NBP2-21790] - Surface staining of human peripheral blood leukocytes using anti-CD148 (MEM-CD148/05) biotin.

Applications for DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DEP-1/CD148

CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Alternate Names

CD148, DEP1, HPTP-eta, PTPRJ

Entrez Gene IDs

5795 (Human)

Gene Symbol

PTPRJ

Additional DEP-1/CD148 Products

Product Documents for DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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