Desmin Antibody (6O9O4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33531
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 6O9O4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 371-470 of human Desmin (P17661).
Sequence:
EEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL
Sequence:
EEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Desmin Antibody (6O9O4)
Immunohistochemistry: Desmin Antibody (6O9O4) [NBP3-33531] -
Immunohistochemistry: Desmin Antibody (6O9O4) [NBP3-33531] - Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using Desmin Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Western Blot: Desmin Antibody (6O9O4) [NBP3-33531] -
Western Blot: Desmin Antibody (6O9O4) [NBP3-33531] - Western blot analysis of various lysates using Desmin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry: Desmin Antibody (6O9O4) [NBP3-33531] -
Immunohistochemistry: Desmin Antibody (6O9O4) [NBP3-33531] - Immunohistochemistry analysis of paraffin-embedded Human smooth muscle tissue using Desmin Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.Applications for Desmin Antibody (6O9O4)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Desmin
Alternate Names
CMD1I, DES
Gene Symbol
DES
Additional Desmin Products
Product Documents for Desmin Antibody (6O9O4)
Product Specific Notices for Desmin Antibody (6O9O4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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