Desmin Antibody (OTI4G1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70565

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4G1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Desmin Antibodies »

Product Specifications

Immunogen

Human fragment corresponding to amino acids 104-391 of human DES (NP_001918) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Desmin Antibody (OTI4G1) - Azide and BSA Free

Western Blot: Desmin Antibody (OTI4G1)Azide and BSA Free [NBP2-70565]

Western Blot: Desmin Antibody (OTI4G1)Azide and BSA Free [NBP2-70565]

Western Blot: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Desmin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Desmin.
Immunohistochemistry: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565]

Immunohistochemistry: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565]

Immunohistochemistry: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565] - Staining of paraffin-embedded Human lymphoma tissue using anti-Desmin mouse monoclonal antibody.
Flow Cytometry: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565]

Flow Cytometry: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565]

Flow Cytometry: Desmin Antibody (OTI4G1) - Azide and BSA Free [NBP2-70565] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Desmin antibody, and then analyzed by flow cytometry.
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Applications for Desmin Antibody (OTI4G1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Desmin

Desmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle.DE-U-10 labels desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas) (Debus E et al.).

Alternate Names

CMD1I, DES

Gene Symbol

DES

Additional Desmin Products

Product Documents for Desmin Antibody (OTI4G1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Desmin Antibody (OTI4G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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