Desmin Antibody (RD301) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97707
![Immunocytochemistry/ Immunofluorescence: Desmin Antibody (RD301) - BSA Free [NBP1-97707]](https://resources.bio-techne.com/images/products/Desmin-Antibody-RD301-Immunocytochemistry-Immunofluorescence-NBP1-97707-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Desmin Antibody (RD301) - BSA Free [NBP1-97707]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Canine, Chicken, Hamster, Rabbit, Zebrafish
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # RD301
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal extract of Chicken gizzard.
Reactivity Notes
Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.
Specificity
This antibody reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Scientific Data Images for Desmin Antibody (RD301) - BSA Free
Immunocytochemistry/ Immunofluorescence: Desmin Antibody (RD301) - BSA Free [NBP1-97707]
Immunocytochemistry/Immunofluorescence: Desmin Antibody (RD301) [NBP1-97707] - Immunofluorescence staining of a 7 days old zebrafish embryoApplications for Desmin Antibody (RD301) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:100-1:200
Western Blot
1:100-1:500
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry on permeabilized cells and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH 7.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Desmin
Alternate Names
CMD1I, DES
Gene Symbol
DES
Additional Desmin Products
Product Documents for Desmin Antibody (RD301) - BSA Free
Product Specific Notices for Desmin Antibody (RD301) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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