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Desmocollin-2 Antibody (7G6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54519

Novus Biologicals, part of Bio-Techne
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NBP2-54519-100ug
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7G6

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Human Desmocollin-2 extracellular domain

Reactivity Notes

Others not known.

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53378).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Desmocollin-2 Antibody (7G6) - Azide and BSA Free

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519]

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519]

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519] - Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2 Antibody (7G6).
Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519]

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519]

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519] - Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2/3 Monoclonal Antibody (7G6).

Applications for Desmocollin-2 Antibody (7G6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Desmocollin-2

Desmocollin 2 is encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11. Alternative splicing results in two transcript variants encoding distinct isoforms.

Alternate Names

ARVD11, CDHF2, Desmocollin2, DG2, DSC2

Gene Symbol

DSC2

Additional Desmocollin-2 Products

Product Documents for Desmocollin-2 Antibody (7G6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Desmocollin-2 Antibody (7G6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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