Desmoglein-3 Antibody (5G11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80694

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-80694
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Catalog #
BSA Free
NB100-1643
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Dot Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G11

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Desmoglein-3 Antibodies »

Product Specifications

Immunogen

Human Desmoglein 3 recombinant protein [UniProt# P32926]

Localization

Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Desmoglein-3 Antibody (5G11) - Azide and BSA Free

Immunocytochemistry: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694]

Immunocytochemistry: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694]

Immunocytochemistry: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694] - Analysis of Desmoglein-3 on human skin cancer. NB100-1643 was used at a dilution of 1:200. Image from the standard format of this antibody.
Flow Cytometry: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694]

Flow Cytometry: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694]

Flow Cytometry: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Desmoglein-3 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694]

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694]

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5G11) - Azide and BSA Free [NBP2-80694] - Analysis of Desmoglein-3 in A-431 cells. Image from the standard format of this antibody.

Applications for Desmoglein-3 Antibody (5G11) - Azide and BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 15588331)

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmoglein-3

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Alternate Names

CDHF6, Desmoglein3, DSG3

Gene Symbol

DSG3

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (5G11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Desmoglein-3 Antibody (5G11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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