Desmoglein-3 Antibody (5G11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07872
![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (5G11) [NBP3-07872]](https://resources.bio-techne.com/images/products/Desmoglein-3-Antibody-5G11-Immunohistochemistry-Paraffin-NBP3-07872-img0001.jpg "Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (5G11) [NBP3-07872]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5G11
Concentration
0.2 mg/ml
Product Summary for Desmoglein-3 Antibody (5G11)
Immunogen
Recombinant human Desmoglein-3 extracellular protein fragment (Uniprot: P32926)
Localization
Cell surface
Specificity
Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.
Marker
Squamous Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08408)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Desmoglein-3 Antibody (5G11)
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (5G11) [NBP3-07872]
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (5G11) [NBP3-07872] - Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).Applications for Desmoglein-3 Antibody (5G11)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Desmoglein-3
Additional Desmoglein-3 Products
Product Documents for Desmoglein-3 Antibody (5G11)
Product Specific Notices for Desmoglein-3 Antibody (5G11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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