Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DSG3/1535

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Desmoglein-3 Antibodies »

Product Specifications

Immunogen

Recombinant human Desmoglein-3 extracellular protein fragment (Uniprot: P32926)

Localization

Cell Surface

Specificity

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.

Marker

Squamous Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07873).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free [NBP3-08409]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free [NBP3-08409]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free [NBP3-08409] - Formalin-fixed, paraffin-embedded human Lung Squamous Cell Carcinoma stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free [NBP3-08409]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free [NBP3-08409]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free [NBP3-08409] - Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

Applications for Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Desmoglein-3

Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Alternate Names

CDHF6, Desmoglein3, DSG3

Gene Symbol

DSG3

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Desmoglein-3 Antibody (DSG3/1535) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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