DET1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13914

Novus Biologicals, part of Bio-Techne
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NBP2-13914
NBP2-13914-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DET1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: THWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVNIRGRLFERFFVLLHITNVAANGEHLNRECS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DET1 Antibody

Immunocytochemistry/ Immunofluorescence: DET1 Antibody [NBP2-13914]

Immunocytochemistry/ Immunofluorescence: DET1 Antibody [NBP2-13914]

Immunocytochemistry/Immunofluorescence: DET1 Antibody [NBP2-13914] - Staining of human cell line A549 shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DET1 Antibody [NBP2-13914]

Immunohistochemistry-Paraffin: DET1 Antibody [NBP2-13914]

Immunohistochemistry-Paraffin: DET1 Antibody [NBP2-13914] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for DET1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DET1

DET1, also known as DET1 homolog, consists of a 63.8 kDa and a 65.1 kDa isoform and is involved in the ubiquitination of target proteins as well as their consequent degradation. This protein is found in large quantities in the ovary and in small quantities in some lymphoid organs. There is currently no research with this protein and its effect on diseases. In antigen processing, immune system, and upiquitin mediated proteolysis pathways, the protein interacts with DDA1, DDB2, RFWD2, RBX1, and CUL4A.

Alternate Names

de-etiolated 1, de-etiolated homolog 1 (Arabidopsis), De-etiolated-1 homolog, DET1 homolog, FLJ10103, MGC126156, MGC126157

Gene Symbol

DET1

Additional DET1 Products

Product Documents for DET1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DET1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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