DFF40/CAD Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77009

Novus Biologicals, part of Bio-Techne
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NBP1-77009
NBP1-77009-0.025mg
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all DFF40/CAD Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD. The immunogen is located within amino acids 190 - 240 of CAD. Amino Acid Squence: YNGSYFDRGAEASSRLCT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Human (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DFF40/CAD Antibody - BSA Free

DFF40/CAD Antibody - BSA Free

Immunohistochemistry: DFF40/CAD Antibody - BSA Free [NBP1-77009] -

Immunohistochemistry: DFF40/CAD Antibody - BSA Free [NBP1-77009] - Immunohistochemistry of DFF40/CAD in mouse kidney tissue with DFF40/CAD antibody at 2 ug/mL.
Western Blot: DFF40/CAD AntibodyBSA Free [NBP1-77009]

Western Blot: DFF40/CAD AntibodyBSA Free [NBP1-77009]

Western Blot: DFF40/CAD Antibody [NBP1-77009] - Analysis of 50 ug of murine lung tissue lysate with anti-CAD (I17) at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-77009]

Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-77009]

Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-77009] - Staining of CAD in mouse kidney tissue with CAD antibody at 5 ug/ml.
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Applications for DFF40/CAD Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 ug/ml
Application Notes
A 40 kDa band should be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DFF40/CAD

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Long Name

DNA Fragmentation Factor, Subunit beta

Alternate Names

CAD, CPAN, DFF2, DFFB

Gene Symbol

DFFB

UniProt

O54788 (Mouse)

Additional DFF40/CAD Products

Product Documents for DFF40/CAD Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DFF40/CAD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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