DFF45/ICAD Antibody (3A11)

Novus Biologicals, part of Bio-Techne | Catalog # H00001676-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DFF45/ICAD Antibodies »

Product Specifications

Immunogen

DFFA (NP_004392.1, 231 a.a. ~ 331 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Specificity

DFFA - DNA fragmentation factor, 45kDa, alpha polypeptide (3A11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DFF45/ICAD Antibody (3A11)

Western Blot: DFF45/ICAD Antibody (3A11) [H00001676-M05]

Western Blot: DFF45/ICAD Antibody (3A11) [H00001676-M05]

Western Blot: DFF45/ICAD Antibody (3A11) [H00001676-M05] - Analysis of DFFA expression in transfected 293T cell line by DFFA monoclonal antibody (M05), clone 3A11. Lane 1: DFFA transfected lysate (Predicted MW: 36.5 KDa). Lane 2: Non-transfected lysate.
Proximity Ligation Assay: DFF45/ICAD Antibody (3A11) [H00001676-M05]

Proximity Ligation Assay: DFF45/ICAD Antibody (3A11) [H00001676-M05]

Proximity Ligation Assay: DFF45/ICAD Antibody (3A11) [H00001676-M05] - Analysis of protein-protein interactions between CASP3 and DFFA. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-DFFA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction c
Sandwich ELISA: DFF45/ICAD Antibody (3A11) [H00001676-M05] - Detection limit for recombinant GST tagged DFFA is 0.1 ng/ml as a capture antibody.

Applications for DFF45/ICAD Antibody (3A11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DFF45/ICAD

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Long Name

DNA Fragmentation Factor, Subunit alpha

Alternate Names

DFF1, DFFA, ICAD

Entrez Gene IDs

1676 (Human)

Gene Symbol

DFFA

UniProt

NP_004392 (Human)

Additional DFF45/ICAD Products

Product Documents for DFF45/ICAD Antibody (3A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DFF45/ICAD Antibody (3A11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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