DFF45/ICAD Antibody (S09-4J6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15022

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15022-100ul
NBP3-15022-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-4J6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DFF45/ICAD Antibodies »

Product Summary for DFF45/ICAD Antibody (S09-4J6)

Immunogen

A synthetic peptide of human DFF45/ICAD (Uniprot#: O00273)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DFF45/ICAD Antibody (S09-4J6)

Western Blot: DFF45/ICAD Antibody (S09-4J6) [NBP3-15022]

Western Blot: DFF45/ICAD Antibody (S09-4J6) [NBP3-15022]

Western Blot: DFF45/ICAD Antibody (S09-4J6) [NBP3-15022] - Western blot detection of DFF45/ICAD in Rat Brain, MCF7, C6 cell lysates using DFF45/ICAD Rabbit mAb (1:1000 diluted). Predicted band size: 37KDa. Observed band size: 45KDa.

Applications for DFF45/ICAD Antibody (S09-4J6)

Application
Recommended Usage

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DFF45/ICAD

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

DNA Fragmentation Factor, Subunit alpha

Alternate Names

DFF1, DFFA, ICAD

Gene Symbol

DFFA

Additional DFF45/ICAD Products

Product Documents for DFF45/ICAD Antibody (S09-4J6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DFF45/ICAD Antibody (S09-4J6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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