Dfna5 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92914

Novus Biologicals, part of Bio-Techne
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NBP2-92914-0.02ml
NBP2-92914-0.1ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Dfna5 Antibodies »

Product Summary for Dfna5 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Dfna5 (NP_004394.1). MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dfna5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Dfna5 Antibody - Azide and BSA Free [NBP2-92914]

Immunocytochemistry/ Immunofluorescence: Dfna5 Antibody - Azide and BSA Free [NBP2-92914]

Immunocytochemistry/Immunofluorescence: Dfna5 Antibody [NBP2-92914] - Immunofluorescence analysis of PC-12 cells using Dfna5 antibody (NBP2-92914) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Dfna5 Antibody - Azide and BSA Free [NBP2-92914]

Immunohistochemistry-Paraffin: Dfna5 Antibody - Azide and BSA Free [NBP2-92914]

Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-92914] - Human pancreatic panniculitis using DFNA5/GSDME Rabbit pAb at dilution of 1:50 (40x lens).
Western Blot: Dfna5 AntibodyAzide and BSA Free [NBP2-92914]

Western Blot: Dfna5 AntibodyAzide and BSA Free [NBP2-92914]

Western Blot: Dfna5 Antibody [NBP2-92914] - Western blot analysis of extracts of Jurkat cells, using Dfna5 antibody (NBP2-92914) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Applications for Dfna5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dfna5

Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

deafness, autosomal dominant 5, DFNA5, deafness associated tumor suppressor, gasdermin-E, ICERE1, ICERE-1, Inversely correlated with estrogen receptor expression 1, nonsyndromic hearing impairment protein, non-syndromic hearing impairment protein 5

Gene Symbol

GSDME

Additional Dfna5 Products

Product Documents for Dfna5 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dfna5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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