DGAT1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-41487

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Block/Neutralize, Flow Cytometry, Immunohistochemistry, Inhibition Activity, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all DGAT1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region (within residues 200-300) of human DGAT1. [Swiss-Prot# O75907]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32326330).

Localization

Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGAT1 Antibody - BSA Free

Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.
Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NB110-41487]

Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NB110-41487]

Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-DGAT1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 Antibody [NB110-41487] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Pictured is protein abundance of DGAT1. In the figure panel, the data is expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blot shown inset above the figure panel. DGAT = diacylglycerol acyltransferase; AU = arbitrary units. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.

Applications for DGAT1 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 23668758)

Immunocytochemistry/ Immunofluorescence

1:40-1:100

Western Blot

2 ug/ml
Application Notes
In Western Blot, a band is seen at ~55 kDa. In ICC/IF, staining was observed in the endoplasmic reticulum and cytoplasm of HepG2 cells. This antibody is not applicable for IHC-paraffin embedded sections.

Reviewed Applications

Read 1 review rated 3 using NB110-41487 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DGAT1

Anabolic signals such as glucose, insulin etc. enhance the transcription of lipogenic enzymes responsible for converting excessive carbohydrates into triglycerides, whereas, DGAT1 (diacylglycerol O-acyltransferase 1) is an enzyme that facilitates the conversion of fatty acids to triglycerides. DGAT1 is a multi-pass membrane protein localized in endoplasmic reticulum which catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2, it is not essential for survival, and is involved in VLDL assembly. In liver, DGAT1 plays a key role in esterifying exogenous fatty acids to glycerol, and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. Moreover, it acts as the major acyl-CoA retinol acyltransferase (ARAT) in skin tissues, wherein, it maintains the retinoid homeostasis for preventing retinoid toxicity responsible for skin and hair disorders.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Gene Symbol

DGAT1

Additional DGAT1 Products

Product Documents for DGAT1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DGAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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