DGCR14 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84258

Novus Biologicals, part of Bio-Techne
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NBP1-84258
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DGCR14 Antibodies »

Product Summary for DGCR14 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASASSLLLPAASRPPRKREAGEAGAATSKQRVLDEEEYIEGLQTVIQRDFFPDVEKLQAQKEYLEAEENGDLERMRQIAIKFGSALGKMSREPPPPYVTPATFETPEVHAGTGVVGNKPRPRGRGLEDGEAGEEEEKEPLPSLDV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DGCR14 Antibody

Western Blot: DGCR14 Antibody [NBP1-84258]

Western Blot: DGCR14 Antibody [NBP1-84258]

Western Blot: DGCR14 Antibody [NBP1-84258] - Analysis in control (vector only transfected HEK293T lysate) and DGCR14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DGCR14 Antibody [NBP1-84258]

Immunocytochemistry/ Immunofluorescence: DGCR14 Antibody [NBP1-84258]

Immunocytochemistry/Immunofluorescence: DGCR14 Antibody [NBP1-84258] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258] - Staining of human cerebral cortex, colon, liver and testis using Anti-DGCR14 antibody NBP1-84258 (A) shows similar protein distribution across tissues to independent antibody NBP1-84257 (B).
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Applications for DGCR14 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DGCR14

DGCR14, also known as DiGeorge syndrome critical region 14, is a 52.6 kDa, 476 amino acid protein that exists in the region thought to be responsible for multiple developmental disorders. Current research is studying the involvement of the protein in diseases and disorders such as DiGeorge syndrome, schizophrenia, velocardiofacial syndrome, and faces syndrome. The protein may be involved in mRNA splicing pathways where it interacts with PFDN1, VIM, TTC14, GNB2L1, and FRA10AC1.

Alternate Names

DGCR13, DGS-HDGS-I, DGSIDGSH, DiGeorge syndrome critical region 13, DiGeorge syndrome critical region 14, DiGeorge syndrome critical region gene 13, DiGeorge syndrome critical region gene 14, DiGeorge syndrome gene H, DiGeorge syndrome gene I, DiGeorge syndrome protein H, ES2DiGeorge syndrome critical region gene DGSI, Es2el, Protein DGCR13, protein DGCR14, Protein ES2

Gene Symbol

ESS2

Additional DGCR14 Products

Product Documents for DGCR14 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DGCR14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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