Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DGCR14 Antibodies »

Product Summary for DGCR14 Antibody

Immunogen

The immunogen recognized by the antibody maps to a region between residue 426 to 476 of human DiGeorge Syndrome Critical Region Gene 14 using the numbering given in entry NP_073210.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DGCR14 Antibody

Western Blot: DGCR14 Antibody [NBP2-36530]

Western Blot: DGCR14 Antibody [NBP2-36530]

Western Blot: DGCR14 Antibody [NBP2-36530] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DGCR14 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DGCR14 Antibody [NBP2-36530]

Immunoprecipitation: DGCR14 Antibody [NBP2-36530]

Immunoprecipitation: DGCR14 Antibody [NBP2-36530] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated DGCR14, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for DGCR14 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:500 - 1:2500
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DGCR14

DGCR14, also known as DiGeorge syndrome critical region 14, is a 52.6 kDa, 476 amino acid protein that exists in the region thought to be responsible for multiple developmental disorders. Current research is studying the involvement of the protein in diseases and disorders such as DiGeorge syndrome, schizophrenia, velocardiofacial syndrome, and faces syndrome. The protein may be involved in mRNA splicing pathways where it interacts with PFDN1, VIM, TTC14, GNB2L1, and FRA10AC1.

Alternate Names

DGCR13, DGS-HDGS-I, DGSIDGSH, DiGeorge syndrome critical region 13, DiGeorge syndrome critical region 14, DiGeorge syndrome critical region gene 13, DiGeorge syndrome critical region gene 14, DiGeorge syndrome gene H, DiGeorge syndrome gene I, DiGeorge syndrome protein H, ES2DiGeorge syndrome critical region gene DGSI, Es2el, Protein DGCR13, protein DGCR14, Protein ES2

Gene Symbol

ESS2

UniProt

NP_073210.1 (Human)

Additional DGCR14 Products

Product Documents for DGCR14 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DGCR14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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