DGK-epsilon Antibody (7E1)
Novus Biologicals, part of Bio-Techne | Catalog # H00008526-M03
![Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]](https://resources.bio-techne.com/images/products/DGK-epsilon-Antibody-7E1-Western-Blot-H00008526-M03-img0009.jpg "Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Lambda Clone # 7E1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DGKE (NP_003638, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEF
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
DGKE - diacylglycerol kinase, epsilon 64kDa
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Lambda
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for DGK-epsilon Antibody (7E1)
![Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]](https://resources.bio-techne.com/images/products/DGK-epsilon-Antibody-7E1-Western-Blot-H00008526-M03-img0008.jpg "Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]")
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in Raw 264.7 (Cat # L024V1).![Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]](https://resources.bio-techne.com/images/products/DGK-epsilon-Antibody-7E1-Western-Blot-H00008526-M03-img0006.jpg "Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]")
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in HeLa (Cat # L013V1).![Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]](https://resources.bio-techne.com/images/products/DGK-epsilon-Antibody-7E1-Western-Blot-H00008526-M03-img0007.jpg "Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]")
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in PC-12 (Cat # L012V1).Applications for DGK-epsilon Antibody (7E1)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23542698).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DGK-epsilon
Long Name
Diacylglycerol Kinase, epsilon 64kDa
Alternate Names
DAGK5, DAGK6, DGKE, DGKepsilon
Entrez Gene IDs
8526 (Human)
Gene Symbol
DGKE
OMIM
601440 (Human)
UniProt
Additional DGK-epsilon Products
Product Documents for DGK-epsilon Antibody (7E1)
Product Specific Notices for DGK-epsilon Antibody (7E1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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